Users can install the ScanLater Western Blot Detection Cartridge in the SpectraMax Paradigm SystemMicroplate readers read biological, chemical or physical events that occur on a microtiter plate. The cuvette must be in place when you read the plate. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Microplate Readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Page 28 Chapter 5: Read Modes and Read Types 1. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SAN JOSE, CA — Molecular Devices, LLC. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 316 grating motor driver faulty. Blank subtraction and standard curve fitting were done automatically by the software. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. With optimized reagents, option to operate the. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. The drawing shows a standard microplate of 15 mm height covered with a lid. We would like to show you a description here but the site won’t allow us. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. 0 out of 5 $ 11,999. Table of Contents. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Omnia® Compatible Microplate Reader Documentation Version No. 305 reference level saturation. A: Microplate Preparation. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Measuring platelet aggregation with a microplate reader. Detailed specifications of each model reader are reported in Section. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Unlock your i3/i3x instrument after shipment. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. western blot membrane detection in a multimode microplate reader platform. 303 unable to cal dark current. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 1) Select the correct plate and the wells that you want to measure. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 8 0112-0126D ComputerIntegration. Avoid losing high signal. A. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Please refer to the Obsolete Microplate Reader Models for more information. B: Selection of Excitation Wavelength. Use the provided 3. 0 out of 5 $ 9,995 $ 9,495. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Devices’ StakMax® Microplate Stacker in a matter of minutes and . For more sample throughput, both readersSupplier: Molecular Devices. Italic and boldface type are used for emphasis. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. 100 fmol 1536-well (8 µL) 0. The SpectraMax® ABS Plus Microplate Reader can run . SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. SpectraMax Spectrofluorometers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The 2-channel configuration is available with 0, 2, or 4 injectors. . 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. com 4. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Note that this procedure is only for the SpectraMax L model. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 305 reference level saturation. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. It can run samples based on pre-defined. SAN JOSE, CA — Molecular Devices, LLC. In addition, the SpectraMax® iD5 can be expanded to include bottom. Add to Cart. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The advanced optical and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Products : General, Microplate Readers. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Request a Quote!Introduction. View the Molecular Devices website. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The chamber contains springs that position the cuvette in the proper alignment for a read. Published Dec 16, 2020 | Updated Dec 16, 2020. Fluorescence Microplate Readers. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. We use Spectramax M5 microplate reader in our lab. their consistent performance, reliability and durability. Figure 3. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Selection of Water Constant in PathCheck settings. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. no. Step 2: Launch SoftMax Pro Software. Products. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. 76358-624. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. Molecular Devices SpectraMax M2E Microplate Reader. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Figure 6: Transcreener ADP2 FI assay principle. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 1. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Fully tested with validations, warranty. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. This pathlength correction is accomplished only with the use of. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Create custom fluorescence assays without having to purchase expensive filter sets. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. For readers without a cuvette port, these options are not shown in the software. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Maximum signal with lowest background. Detailed specifications of each model reader are reported in Section 7, “Specifications”. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. 012 fmol 0. 314 low lamp level warning. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. If it looks fine, then proceed. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. . . 40266 Email: drugdiscoverytech@lifetech. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The advanced optical and. both cuvette-based and microplate reader applications on the same instrument. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Absorbance (optical density, OD) was read at 570 nm and 605. begin reading microplates with seven mouse clicks. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. 470 nm). SpectraMax 250 User. 304 signal level saturation. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraDrop microplate was read on the SpectraMax ® Paradigm ® Multi-Mode Microplate Reader using the Fluorescence Intensity detection cartridge (FI-FLRH) with fluorescein filters, or Tunable Wavelength Selection (TUNE) cartridge with signal/ background-optimized wavelengths. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Example: [Drawer]. Italic and boldface type are used for emphasis. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. Five preferred detection modes. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. e. Transcreener ® ADP. Tecan Infinite Microplate Reader. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. We would like to show you a description here but the site won’t allow us. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Use the provided 3. Microplate Readers. 5 12. Also see:. . flat meniscus), but is approx 15 mL. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Find our technical support information along with contact details. Reader modes are customized to your specific applications with options to upgrade easily. Introduction. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Prerequisites: SoftMax Pro 7. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Italic and boldface type are used for emphasis. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. SpectraMax Glo Steady-Luc Reporter Assay Kit. Results are shown in Table 1. Figure 1. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. 7°C (gradient heating design). The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Learn more about its features, specifications, and applications in this user. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Endpoint, kinetic, spectrum. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. 1. The price range of a reader starts at 2. Temperature control. Microplate automation and detection instruments for walk away operation and high quality results. Our absorbance plate readers feature our PathCheck Sensor technology and. Step 1: Open the SoftMax Pro IMAP FP protocol. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. both cuvette-based and microplate reader applications on the same instrument. View the Molecular Devices website. Time-. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. These lab workhorses are used in drug discovery. Wide range of applications. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The plate reader can measure multiple readouts, including UV and visible absorbance. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The plate reader optics are tunable for absorbance. Unparalleled performance on a personalized platform. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. 6% for the on-board optics (monochromators). 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Turn the reader off by pulling the power cord. Offer Ends In: 2 Days 10: 40: 19. Supplier: Molecular Devices. Examples: “Press carefully to engage,” “ Do not press down. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Download PDF. We would like to show you a description here but the site won’t allow us. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Results are shown in Table 1. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Find regional support. 1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 5 87. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Example: [Drawer]. . For readers without a cuvette port, simply check the box next to ‘PathCheck’. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Materials. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. With optimized reagents, option to operate the. Open the top cover of the instrument. 6 for further experiment, so I'm confused which i would choose for OD value determination. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. Detection modalities include absorbance (UV-Vis Abs) and. Examples: “Press carefully to engage,” “ Do not. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. An assay plate containing a 15-point standard curve was read on the SpectraMax. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Temperature control. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. More Product Information. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Plate chamber design of the SpectraMax category 1 microplate readers. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. A preconfigured protocol is available in the software to. Molecular Interactions. In addition, the SpectraMax iD5 can be expanded to. It also integrates with the. Type:Spectrophotometer. This unlocks the transport slide. microplate reader. . Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Fill a clean plate with water. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Multimode Microplate Reader. 58% for the detection module and 2. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Using the. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Wide range of applications The VersaMax reader covers. The plate. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Each sample has a discrete sample beam and reference beam so that each well is measured directly. On the back, in the right hand top corner,. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Figure 1. HTRF-compatible SpectraMax Multi-Mode Readers. . Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The following document is intended to demonstrate setup of this instrument. SpectraMax iD5 Multi-Mode Microplate Reader. 003 fmol 0. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Use similar settings for other Molecular Devices readers with fluorescence detection mode.